Concepts and Applications of PRP, CPT, and PRF

Concepts and Applications of PRP, CPT, and PRF

Ⅰ. PRP serum separation gel (figure 1)

The density of PRP (platelet-rich plasma) serum separation gel is approximately 1.055-1.065, and there is sodium citrate anticoagulant in the tube. After centrifugation, the blood will be layered: the upper layer is plasma, the middle layer is PRP, the lower layer is blood cells, and a layer of serum separation gel separates each layer. A sterile syringe can be used to extract the PRP from the middle layer, and avoid touching serum separation gel and blood cells layer during operation.

PRP serum separation gel tube: Through precise centrifugation and the effect of serum separation gel, the purity and concentration of PRP can be improved, resulting in a higher platelet content in the separated PRP, while also preserving biologically active substances such as growth factors in platelets.

Mainly used for: Platelet function testing, growth factor testing, inflammatory factor testing, cytokine testing, etc.

Aesthetic and plastic surgery field: Facial rejuvenation, hair regeneration.

Orthopedic field: Fracture healing, treatment of joint diseases, repair of tendon and ligament injuries.

Oral field: Dental implant surgery, oral soft tissue repair.

Wound repair: PRP can accelerate wound healing, reduce pain, and minimize scar formation, whether it is for acute traumatic wounds or chronic non-healing wounds such as diabetic foot ulcers and pressure ulcers.

Ⅱ. CPT serum separation gel (figure 2)

The density of CPT (monocyte) serum separation gel is approximately 1.080-1.085. When the separation solution Ficoll Hypaque (with a density of 1.077) is added, and there is sodium citrate anticoagulant in the tube, mononuclear cells (such as lymphocytes and monocytes) can be accurately separated after centrifugation.

Mainly used for: Lymphocyte immune function testing, HLA or residual leukemia gene testing, and immune cell therapy provide a standardized method for further extraction of monocytes for clinical diagnostic specimen preparation and cellular immunotherapy.

Ⅲ. PRF

PRF (platelet-rich fibrin) is obtained after centrifugation.

For a 10ml additive-free glass tube, centrifugation at 2700rpm for 3 minutes gets I-PRF, and centrifugation at 2700rpm for 12 minutes gets PRF clot (figure 3).

Mainly used for: PRF is widely used in the fields of cosmetology and medicine, such as filling tear troughs, neck lines, and apple cheeks, as well as promoting wound healing and tissue regeneration. It has shown potential in bone tissue healing and soft tissue repair, but its clinical efficacy may vary depending on the application scenarios.

The differences between PRF and PRP: Although PRP is also a platelet concentrate extracted by centrifugation, the preparation process of PRF is simpler and can form a fibrin clot without the additional addition of thrombin. PRF may perform better in terms of the long-term release of growth factors, but its clinical efficacy may not be as significant as that of PRP in certain specific applications.